Liverpool’s defence of their Champions League crown will continue with a two-legged tie against Atletico Madrid in the last 16, while Manchester City take on Real Madrid.
Chelsea will be involved in another heavyweight tie, as they face German giants Bayern Munich, and Tottenham – the beaten finalists from 2019 – must try and overcome RB Leipzig.
Barcelona have regularly expressed a desire to get back on top of the podium in European competition and continue their adventure against Napoli.
Paris Saint-Germain, with Neymar and Kylian Mbappe leading the charge, will take in a tough test against Borussia Dortmund.
Cristiano Ronaldo, as the competition’s all-time leading goalscorer, can add to his stunning collection of continental efforts in a meeting with Lyon, while Atalanta’s clash with Valencia completes the first knockout stage.
Liverpool claimed the sixth European Cup success of their illustrious history last season and are once again looking to complement a Premier League title bid with another continental charge.
Their memorable triumph over Spurs back on June 1 came at Wanda Metropolitano, and Jurgen Klopp’s side will be heading back to that venue when taking on Atletico Madrid.
Manchester City, who are looking for a first continental success, are also set to pay a visit to the Spanish capital.
Pep Guardiola’s men face the most unenviable of tests against 13-time winners Real Madrid, who find themselves in this position after finishing as runners-up in their group.
Chelsea are set to take in a reunion with Bayern Munich, having overcome them to claim the Champions League crown back in 2012, while Tottenham – with two-time winner Jose Mourinho now calling the shots – complete the English contingent and also face Bundesliga opposition in the form of RB Leipzig.
Lionel Messi and Co are determined to take a famous trophy back to Camp Nou, and the six-time Ballon d’Or winner will want to be at his talismanic best when taking on a dangerous Napoli side.
Ronaldo has previously added to his medal collection in this competition with Manchester United and Real Madrid, with Juventus’ next step in a bid to end their barren run in the Champions League seeing them tackle French foes in the form of Lyon.
PSG boast plenty of world-class winners within their squad and will want to see potential turned into something more tangible, starting against Borussia Dortmund in the last 16.
Elsewhere, surprise package Atalanta – who are gracing this stage for the first time – will have their sights set on another prized scalp when they lock horns with Valencia.
The first legs in a series of eagerly-anticipated encounters will take place on February 18/19 and 25/26.
The return dates are due to be held on March 10/11 and 17/18.
Champions League last-16 draw in full:
Borussia Dortmund vs Paris Saint-Germain
Real Madrid vs Manchester City
Atalanta vs Valencia
Atletico Madrid vs Liverpool
Chelsea vs Bayern Munich
Lyon vs Juventus
Tottenham vs RB Leipzig
Napoli vs Barcelona